Fig. S3.

VACV is not a direct inhibitor of necroptosis. (A) L929 cells were infected with WTVACV at an MOI of 5. At 5 HPI, cells were treated with 50 µM zVAD and subsequently treated with 20 µg/mL of mouse TNF-α at 6 HPI. Cell viability was measured by Sytox exclusion at 11 HPI by taking the average percent of viable cells in a total of 10 fields, imaged at 20×. (B) Cells were infected and treated as described above, but were prepared as lysates for Western blot analysis at 11 HPI.