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(A) EcGUVs. Green channel, pyranine; orange channel, Rh-PE. (B) D-F1F0-GUVs after detergent removal. Green channel, pyranine; orange channel, Rh-PE. (C) Incorporation of F1F0 proteins into GUVs. Orange channel, Alexa-647 labeled F1F0-ATPase. (Scale bars: 10 microns.)
