Fig. 2.

Monoclonal antibody-mediated neutralization of p40, but not p40₂, induces death of different mouse cancer cells. KLN (A and E), TRAMP-C2 (B and F), 4T1 (C and G), and Hepa (D and H) cells were treated with mAbs against p40 and p40₂ for 48 h under serum-free conditions followed by monitoring of LDH release (A–D) and MTT [(E–H) *P < 0.01 vs. control]. t-type calcium influx was performed in KLN (I), TRAMP-C2 (J), 4T1 (K), and Hepa (L) cells. t-type calcium influx was measured in the presence of 1 M KCl. TUNEL assay (M) and phycoerythrin-tagged Annexin V staining (N) were performed in KLN, TRAMP-C2, 4T1, and Hepa cells. TUNEL-positive (O) and phycoerythrin-tagged Annexin V-positive (P) cells were counted in 10 different images per group and then plotted as percentages of control. All results are mean ± SD of three different experiments (*P < 0.001 vs. respective controls for TUNEL and Annexin V assays).