Fig. S5.

WGP (whole glucan particle, dispersible) Dectin-1–mediated signaling to NF-κB is inhibited by the CARD9-targeting compounds. (A) Scleroglucan is a more potent ligand than WGP that activates Dectin-1–mediated signaling. NF-κB activation in THP-1 cells reconstituted with Dectin-1 was measured by NF-κB–driven luciferase reporter in the presence or absence of 50 μg/mL WGP or scleroglucan. (B) Cells were stimulated with WGP (50 μg/mL) in the presence or absence of 200 μM BRD5529 or BRD4203. At the 4-h time point, luciferase activity was quantified. RLU, relative light units. For A and B, data represent the mean ± SD of at least six replicates and are representative of two independent experiments. P values were calculated by Student’s t test. (C) Inhibition of Dectin-1–mediated (scleroglucan or WGP stimulated) NF-κB luciferase reporter inhibition by heterologous expression of CTD of CARD9 in wild-type THP-1 cells. Flag-vector plasmid or Flag-CARD9 were used as negative controls, and CTD expression was confirmed by anti-Flag Western blot. Data represent mean ± SD of three replicates and are representative of two independent experiments.