Fig. S1.

Fos plume and distant Fos quantification. (A) Representative coronal slices for each site tested. The pink square indicates the site of the 50× 50 μm box used to count Fos. (B) Fos expression showing the local plume surrounding the microinjection in the OFC hotspot (Top), the OFC/insula coldspot (Middle), or the insula hotspot (Bottom). Radial arms of 50 × 50 μm boxes are centered on the microinjection site, and Fos is sampled in each box to measure local plumes. The individual plume for this animal shows a suppressive plume (blue: 25% less Fos relative to vehicle controls) and an excitatory plume (yellow: 200% increase). (C, Left) Schematic of distant Fos in the rostrodorsal medial shell of the NAc (blue) after microinjections into the rostral orbitofrontal cortex hotspot (purple). (Right) Representative images for each drug condition. (D) Average Fos counts in the accumbens hotspot after vehicle (V), DAMGO (D), or orexin (O) stimulation in the OFC hotspot. (E, Left) Schematic of distant Fos in the caudal NAc (yellow) after microinjections in the caudal insula hotspot (brown). (Right) Representative images of each drug condition. (F) Average Fos counts for the accumbens coldspot after vehicle, DAMGO, or orexin stimulation in the insula hotspot.