Fig. 3.
TMP induces oxidative stress response. OD600 nm and fluorescence were measured during growth of the wt strain, carrying a PsoxS-GFP reporter, in LB or LB TMP (0.08 µg/mL), with or without the addition of thymidine (0.3 mM). Paraquat (0.25 mM) was used as a positive control. After substraction of the autofluorescence background of the control strain without a PsoxS-GFP reporter, the fluorescence ratio between treated and nontreated cultures at OD600 nm = 0.4 was calculated. The mean ± SEM of three independent experiments is presented. *Significant difference of PsoxS expression in TMP-treated mutant cells compared with wt cells (t test, P values < 0.05). +Significant difference of PsoxS expression in thymidine-supplemented cultures compared with cultures without thymidine (t test, P values < 0.05).