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. 2017 Sep 18;114(43):11416–11421. doi: 10.1073/pnas.1707970114

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Fig. S5. — Membrane interactions and activity of Brag2. (A) Hill plot analysis of liposome-bound Brag2 as a function of PIP₂ concentration shown in Fig. 4E. This analysis indicates that PIP₂ lipids bind to Brag2 in a positively cooperative manner. (B) Flotation analysis of the interaction of Brag2 with liposomes containing either PIP₂ or PIP₂/PS lipids as a source of anionic lipids. B, bottom fraction containing unbound proteins; T, top fraction, containing liposome-bound proteins. (C) Analysis of the GEF efficiency of Brag2 on liposomes containing either PIP₂ or PIP₂/PS. (D) Comparison of the GEF efficiencies in solution of wild-type Brag2^Sec7-PH and the K549A-R552A mutant.