Fig. 3.

BORIS is critical for DNA methylation-dependent exon 10 inclusion in PKM. (A and B) ChIP in MCF7 cells transfected with shRNA against BORIS versus shControl using (A) BORIS and (B) RNA Pol II antibody, followed by qRT-PCR relative to input and control IgG (n = 5). (C) qRT-PCR to measure the splicing pattern of PKM gene after down-regulation of BORIS. Indicated exon junction-specific primers were used and normalized with RPS16 and constitutive exon of PKM gene (n = 3). (D) Western blot for PKM1 and PKM2 protein level expression in shBORIS and shControl MCF7 cells. Error bar shows mean values ± SD. As calculated using two-tailed Student’s t test, ***P < 0.001; ns, nonsignificant.