Table S3.
Quantitative binding analysis of TRAF4 variants with receptor peptides
| TRAF4 variants | Peptides | SPR | ITC | Interaction |
| Wild type | GPIbβ (RRLRARARAR) | Kd = 11.3 ± 10.8 | Kd = 46.1 ± 28.4 | Interact |
| GPVI (KRLRHRGRAVQ) | N.D. | Kd = 35.6 ± 14.2 | Interact | |
| F434R | GPIbβ (RRLRARARAR) | Kd = 27.2 ± 21.1 | Kd = 82.6 ± 24.4 | Reduced |
| GPVI (KRLRHRGRAVQ) | N.D. | Kd = 108.2 ± 52.1 | ||
| S357E | GPIbβ (RRLRARARAR) | Kd = 90.6 ± 64.8 | Kd = 95.8 ± 35.6 | Reduced |
| GPVI (KRLRHRGRAVQ) | N.D. | N.D. | ||
| Y366R | GPIbβ (RRLRARARAR) | N.R. | N.R. | Disrupt |
| GPVI (KRLRHRGRAVQ) | N.R. | N.R. | ||
| Y436R | GPIbβ (RRLRARARAR) | N.R. | N.R. | Disrupt |
| GPVI (KRLRHRGRAVQ) | N.R. | N.R. | ||
| E406R | GPIbβ (RRLRARARAR) | N.R. | N.R. | Disrupt |
| GPVI (KRLRHRGRAVQ) | N.R. | N.R. | ||
| N355R | GPIbβ (RRLRARARAR) | N.R. | N.R. | Disrupt |
| GPVI (KRLRHRGRAVQ) | N.R. | N.R. | ||
| Wild type | NOD2 (GPPQKSPATLGLEEL) | N.R. | N.R. | Not interact |
| NOD2′ (ERLARKA) | N.R. | N.R. | Not interact | |
| Wild type | TGFBR1 (ARLTALRIKK) | N.D. | Kd = 115.3 ± 48.2 | Interact |
| Wild type | TGFBR2 (DRLSGRSCSE) | N.D. | N.D. | Interact (low affinity) |
| TGFBR2′ (ARLTAQCVA) | N.D. | N.D. | Interact (low affinity) |
Kd is the dissociation constant in micromolar. Data are the mean values from two independent experiments. N.R. and N.D. indicate no response and not determined, respectively.