Fig. 3.

Transcriptional analysis of Micu2^−/− left ventricle. (A) Transcriptome-wide analysis of Micu2^−/− and wild-type LV tissue (n = 3 mice) revealed enrichment in three gene sets: sarcomere components, stress response genes, and bZIP transcription factors (TF). Bonferroni-corrected P values are plotted. (B) Micu2, Myh7, and Aplnr were three of the most differentially expressed genes in the left ventricle RNA-seq datasets. Fold-change differences in these genes were independently confirmed with quantitative PCR (n = 5, t test). Mean and SD of Micu2^−/− qPCR expression normalized to mean wild-type qPCR expression are plotted.