. 2017 Oct 9;114(43):11386–11391. doi: 10.1073/pnas.1714805114

Table S1.

Genetic characterization of selected transgenic maize lines overexpressing EcPAPR

Event	Characteristics of the standard curves^*					Average 2^ΔCt ± SD^†	Estimated copy number^‡	Transgene segregation (+/−)^§
Event	Genes	Efficiency	R^†	Slope	Intercept	Average 2^ΔCt ± SD^†	Estimated copy number^‡	Transgene segregation (+/−)^§
PE5-B101	GAPDH	1.0024 ± 0.0309	0.9953 ± 0.0064	−3.3175 ± 0.0738	40.7405 ± 0.5947			51/48
	Bar	1.0710 ± 0.0318	0.9901 ± 0.0091	−3.1638 ± 0.0668	41.0330 ± 1.6136	1.1020 ± 0.0410	1
RE3-B101	GAPDH	1.0026 ± 0.0039	0.9968 ± 0.0035	−3.3158 ± 0.0093	40.5855 ± 0.1068			45/51
	Bar	0.9805 ± 0.0390	0.9950 ± 0.0061	−3.3715 ± 0.0972	42.9820 ± 0.863	0.6090 ± 0.0086	1
PE5-B73	ND^¶							29/25
RE3-B73	ND							10/8
PE5-Mo17	ND							9/6
PE5-A654	ND							11/11

The standard curve was generated with the SYBR Green qRT-PCR and used for determination of the transgene copy number.

^{^†}

Ratio of the copy number of the transgene (Bar, selectable marker gene for bialaphos resistance) to the reference gene (GAPDH, glyceraldehyde-3-phosphate dehydrogenase) was calculated using the equation: ratio = 2^(Ctreference ⁻ ^Cttransgene).

^{^‡}

DNA from leaf tissues of PE5-B101 and RE3-B101 were from germinated S₁BC₄ and BC₅ seeds, respectively.

^{^§}

+ and − denote the number of plants that tested positive and negative for the EcPAPR transgene. These transgenic events were characterized at the BC₃ generation with the exception of RE3-B101, which was analyzed at the BC₄ generation.

^{^¶}

ND, not determined.