Table S3.

Reports on transgenic seeds with elevated Met content expressing the Met-rich sunflower seed albumin (SSA) or the 10-kDa δ-zein

Plant	Tissue-specific expression of a Met-rich protein	Sulfur content of transgenic seeds	Refs.
Lupins	Seed-specific expression of an SSA gene	No difference in total S content between control and transgenic seeds Marked differences in the distribution of S between the oxidized (sulfate) and the reduced C-bonded S fraction (cysteine and Met)	12, 13
Chickpea	Seed-specific expression of an SSA gene	Total seed S concentrations were not consistently different between transgenic and control genotypes Transgenic seeds have significantly higher reduced S than the parental control	10
Rice	Grain-specific expression of SSA	S content does not change in the transgenic grain Level of reduced S was not significantly different between control and transgenic lines	11
Maize*	Seed-specific overexpression of its own 10-kDa δ-zein	The null segregant kernels have 0.132% (0.002)†, whereas the transgenic kernels have 0.138% (0.002)† sulfur contents	9

^*This transgenic maize is from our own laboratory stock.

^†The sulfur contents of the maize kernels are shown as means (SD) of three replicates. Statistical analysis performed with the Student’s t test showed that the value of the transgenic kernels is significantly different from the null segregant kernels at P < 0.01.