Table S4.

Composition of the experimental diets used in the chick feeding trials

Ingredient	Amount
	Normal	Reference	PE5
Corn meal, g*	717.5	720.0	720.0
Soy protein, g†	150.0	150.0	150.0
DL-Met, g‡	2.5	—	—
Cellulose, BW200, g	30.0	30.0	30.0
Corn oil, g	30.0	30.0	30.0
Vitamin mixture, g§	10.0	10.0	10.0
Salt mixture, g¶	60.0	60.0	60.0
Total, g	1,000	1,000	1,000

Cornish male chicks were used in a 4-wk feeding trial starting with 5-d-old chicks in triplicate groups of five per diet formulation.

^*Three diet rations with different corn meals were used in the feeding experiment: yellow dent corn supplemented with Met (normal group), null transgenic segregant from event PE5 without synthetic Met supplementation (reference group), and high-Met transgenic event PE5 without synthetic Met supplementation (PE5).

^†Supro isolated soy protein (DuPont) contains 1.1% Met and 1.1% cysteine.

^‡DL-Met was added to the diet at a concentration of 2.5 g/kg (52).

^§Supplied per kilogram of diet: Vitamin A palmitate, 500,000 IU/g, 8 mg; Vitamin D3, 100,000 IU/g, 10 mg; Vitamin E acetate, 500 IU/g, 50 mg; menadione sodium bisulfite, 62.5% menadione, 3 mg; biotin, 1%, 20 mg; cyanocobalamin, 0.1%, 10 mg; folic acid, 2 mg; nicotinic acid, 70 mg; calcium panthothenate, 15 mg; pyridoxine-HCl, 10 mg; riboflavin, 5 mg; thiamine-HCl, 5 mg; sucrose, 9,792 mg.

^¶Supplied per kilogram of diet: CaCO₃, 900 mg; Ca₃(PO₄)₂, 30 g; MgO, 1,002 mg; C₆H₅K₃O₇, 17.28 g; K₂SO₄, 1.65 g; NaCl, 4.5 g; Cu₂(OH)₂CO₃, 13.80 mg; KIO₃, 660 μg; C₆H₅FeO₇, 376.80 mg; MnCO₃, 126 mg; Na₂SeO₃, 420 μg; ZnCO₃, 144 mg; sucrose, 4,006.32 mg.