Fig. S4.

β-carotene supplementation of dams affects lymphoid immunity in the offspring. (A) Intestinal lymphoid follicles were dissected, and isolated cells were subjected to analysis by flow cytometry. T-cell gating strategy and representative analyses of CD45⁺, CD3⁺, CD4⁺, and CD8⁺ cells. SSC-A, Side-scatter area; FSC-A, forward-scatter area. (Magnification: 12×.) (B) Percentage of CD45⁺ cells of isolated cells in offspring from chow diet and BC diet-fed dams. Percentage of (C) CD3⁺CD4⁺CD8⁻ T cells, (D) CD3⁺CD4⁻CD8⁺ T cells, (E) CD3⁺CD4⁻CD8⁻ T cells, and (F) CD3⁻CD19⁺ B cells in isolated cells of offspring from chow diet and BC diet-fed dams. Values indicate mean ± SD of results from four lymphoid follicles from two animals per supplementation group. Threshold of significance was set at *P < 0.05.