Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 1;11:33. doi: 10.1186/s13036-017-0075-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Schematic overview of promoter, UP element-promoter and half UP element-promoter construct designs based on 19 constitutive promoters in E. coli. RNAP binds with −35 (marked in dark green) and −10 (marked in orange) in the constitutive promoter to initiate transcription; a full synthesized UP element (sequence in red), a half synthetic UP element (sequence half in red and half in black) and a consensus upstream sequence (sequence in black) located upstream constitutive promoters provides two extra binding sites (marked in green and light blue) with RNAP-αCTD, one binding site, and no binding sites, respectively