Table 1.

16-Plex PCR primers and the virulence genes detected.

Pathotypes	Target Genes	Primer Sequence (5′–3′)	Product Size (bp)	Concentration (µM)	References
STEC, EPEC	eaeA	eae-F: TCAATGCAGTTCCGTTATCAGTT eae-R: GTAAAGTCCGTTACCCCAACCTG	482	0.1	[36]
	escV	MP3-escV-F: ATTCTGGCTCTCTTCTTCTTTATGGCTG MP3-escV-R: CGTCCCCTTTTACAAACTTCATCGC	544	0.4	[32]
	ent	ent-F: TGGGCTAAAAGAAGACACACTG ent-R: CAAGCATCCTGATTATCTCACC	629	0.4	[32]
Typical EPEC	bfpB	MP3-bfpB-F: GACACCTCATTGCTGAAGTCG MP3-bfpB-R: CCAGAACACCTCCGTTATGC	910	0.1	[32]
STEC	EHEC-hly	hlyEHEC-F: TTCTGGGAAACAGTGACGCACATA hlyEHEC-R: TCACCGATCTTCTCATCCCAATG	688	0.1	[33]
	stx1	MP4-stx1A F: CGATGTTACGGTTTGTTACTGTGACAGC MP4-stx1A-R: AATGCCACGCTTCCCAGAATTG	244	0.2	[32]
	stx2	MP3-stx2A-F: GTTTTGACCATCTTCGTCTGATTATTGAG MP3-stx2A-R: AGCGTAAGGCTTCTGCTGTGAC	324	0.4	[32]
EIEC	ipaH	ipaH-F: GAAAACCCTCCTGGTCCATCAGG ipaH-R: GCCGGTCAGCCACCCTCTGAGAGTAC	437	0.1	[33]
	invE	MP2-invE-F: CGATAGATGGCGAGAAATTATATCCCG MP2-invE-R: CGATCAAGAATCCCTAACAGAAGAATCAC	766	0.2	[37]
EAEC	aggR	MP2-aggR-F: ACGCAGAGTTGCCTGATAAAG MP2-aggR-R: AATACAGAATCGTCAGCATCAGC	400	0.2	[32]
	pic	MP2-pic-F: AGCCGTTTCCGCAGAAGCC MP2-pic-R: AAATGTCAGTGAACCGACGATTGG	111	0.2	[32]
	astA	MP2-astA-F: TGCCATCAACACAGTATATCCG MP2-astA-R: ACGGCTTTGTAGTCCTTCCAT	102	0.4	[32]
ETEC	elt	MP2-LT-F: GAACAGGAGGTTTCTGCGTTAGGTG MP2-LT-R: CTTTCAATGGCTTTTTTTTGGGAGTC	655	0.1	[32]
	estIa	MP4-STIa-F: CCTCTTTTAGYCAGACARCTGAATCASTTG MP4-STIa-R: CAGGCAGGATTACAACAAAGTTCACAG	157	0.4	[32]
	estIb	MP2-STI-F: TGTCTTTTTCACCTTTCGCTC MP2-STI-R: CGGTACAAGCAGGATTACAACAC	171	0.2	[32]
E. coli	uidA	MP2-uidA-F: ATGCCAGTCCAGCGTTTTTGC MP2-uidA-R: AAAGTGTGGGTCAATAATCAGGAAGTG	1487	0.2	[32]

STEC, shiga toxin-producing Escherichia coli; EPEC, enteropathogenic E. coli; EIEC, enteroinvasive E. coli; EAEC, enteroaggregative E. coli; ETEC, enterotoxigenic E. coli.