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. 2017 Oct 13;14(10):1221. doi: 10.3390/ijerph14101221

Figure 3.

Figure 3

Cell viability of immortalized human bronchial epithelial (BEAS-2B) cells treated with cigarette smoke extract (CSE), nicotine section of CSE (CSE-N) and nicotine-free section of CSE (CSE-O). The cells were treated with CSE, CSE-N, CSE-O, CSE-O/N, or nicotine standard for 24 h, and a cell counting kit 8 (CCK-8) assay was used to determine cell viability. (A) CSE; (B) CSE-N; (C) CSE-O; (D) CSE-O/N; (E) nicotine standard; (F) representative photography of cells treated with 4% CSE, CSE-N, CSE-O, CSE-O/N, or nicotine standard (100 μM). The data are presented as the mean ± SD of three independent experiments with triplicate samples; bar: 50 μm.