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. 2017 Oct 24;14(10):1284. doi: 10.3390/ijerph14101284

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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DBP induces the activation of mitogen-activated protein kinase (MAPK)-associated proliferation protein in testicular tissue. The protein levels of mitogen-activated protein kinase kinases1/2 (MEK1/2) and phospho-MEK1/2 (p-MEK1/2) in testicular tissue treated with various concentration of DBP were measured by Western blot. The expression levels were quantified with ImageJ (lower panel; n = 3). GAPDH was run as an internal control. Results are expressed as means ± S.D. ** p < 0.01; * p < 0.05. Scale bar = 100 μm.