Fig. 5.

MiR-224 directly targets AKT1 and MAPK1. a Predicted miR-422a target sequences in the 3′-UTRs of AKT1 and MAPK1, and their mutants containing altered nucleotides in the 3′-UTRs. b Real-time PCR analysis of AKT1 and MAPK1 in the indicated cells. c Western blot analysis of AKT1 and MAPK1, and the their phosphorylation in the indicated cells. d, e Luciferase assay analyses of the indicated cells transfected with the indicated reporters with increasing amounts of miR-422a (10 and 50 nM). Error bars represent mean ± SD from three independent experiments.**p < 0.01