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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Aug 18;68(3):139–149. doi: 10.1002/cm.20471

Fig. 4. MAP kinase inhibitors counteract the inhibition of MEKK1 on MLC20 phosphorylation.

Fig. 4

(A) Three days after adenovirus infection (90 pfu/cell), MEKK1-adenovirus-infected cells were incubated with SB (30 μM), SP (30 μM), and ERK inhibitor (60 μM) for 40 min. Cells were harvested for Western blotting. Membranes were detected by antibodies against p-MLC20 and actin. (B) Permeabilized cells were treated with 1000 nM of calmodulin and MLCK inhibitors for 3 h and then exposed to MAP kinase inhibitors for 40 min. Cell lysates were analyzed by Western blotting. Neither calmodulin or MLCK inhibitors affected the inducement of MAP kinase inhibitors on MLC20 phosphorylation.SB, SB 203580, a p38 inhibitor; SP, SP600125, a JNK inhibitor; ERK in, ERK inhibitory peptide.