5hmC-im in the E17 frontal cortex across groups. Alcohol reduced 5hmC-im in the cortical SVZ/VZ layers (C) compared to Chow (A) and PF controls (B), while no significant change was observed between controls (D). At the SP cortical layer, the only significant alteration detected was an increase in the PF group as compared to both Chow and Alc groups. (A–D) A marked increase of 5hmC-im was observed at the CP region in both the PF and Alc groups as compared to the Chow group, while a significant increment was also evident in the Alc group CP as compared to the PF group. *p < 0.05. N = Chow (5), PF (4), Alc (5). CP (cortical plate); MZ (marginal zone); SP (subplate); SVZ (subventricular zone); VZ (Ventricular zone).