Skip to main content
. Author manuscript; available in PMC: 2018 May 8.
Published in final edited form as: Curr Biol. 2017 Apr 27;27(9):1387–1391. doi: 10.1016/j.cub.2017.03.069

Figure 1. Visualization of Pol II elongation in living embryos.

Figure 1

(A) Schematic representation of MS2-lacZ-PP7 and PP7-MS2-lacZ reporter genes containing hb proximal enhancer and P2 promoter.

(B) Representative snapshots of embryo expressing hb-PP7-MS2-lacZ at the onset of nc 14. Nuclei were visualized with His2Av-eBFP2. Scale bar indicates 5 μm.

(C) Representative trajectory of MS2 and PP7 intensities in an individual nucleus expressing hb-PP7-MS2-lacZ. The time resolution is 9.77 sec (same in all subsequent figures).

(D) Representative snapshots of embryo expressing hb-MS2-lacZ-PP7 at the onset of nc 14. Nuclei were visualized with His2Av-eBFP2. Scale bar indicates 5 μm.

(E) Representative trajectory of MS2 and PP7 intensities in an individual nucleus expressing hb-MS2-lacZ-PP7.

See also Movie S1-3.