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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Acta Biomater. 2017 Jan 23;51:279–293. doi: 10.1016/j.actbio.2017.01.058

Scheme I.

Scheme I

Schematic representation of layer-by-layer (LbL) adsorption of polyelectrolytes based on electrostatic interactions. (i) The substrate (polystyrene well plates) was first modified with polyethylenimine (PEI) and extensive washed. (ii) The positive charged substrate was coated with polysaccharides that shared marine origin (ALG and CHI) using LbL assembly. Between the polyelectrolyte depositions a washing step was performed. (iii) The adhesive properties of ALG/CHI multilayers can be tuned by the addition of ECM proteins, chemical cross-linking or a combination of thereof. (iv) Upon the addition of ALG/CHI on the top of the cell adhesive ALG/CHI multilayers the films became non-adhesive.