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. Author manuscript; available in PMC: 2018 Feb 20.
Published in final edited form as: Chem Res Toxicol. 2017 Jan 19;30(2):715–725. doi: 10.1021/acs.chemrestox.6b00451

Table 2.

Steady State Kinetic Parameters for the Hyperbolic Mixed-type Inhibition of DNA Polymerase by HNLa

pol kcat (min−1) Km (nM) Ki (μM) α β
β 28 (26, 30) 220 (180, 260) 4.0 (2.4, 5.6) 0.55 (0.31, 0.79) 0.20 (0.15, 0.25)
λ 5.2 (4.8, 5.5) 274 (230, 310) 8.3 (4.8, 11.9) 0.51 (0.24, 0.78) 0.07 (0.02, 0.12)
η 7.4 (6.7, 8.0) 390 (330, 450) 20 (7, 34) 0.49 (0.12, 0.85) 0.03 (–0.10, 0.15)
Kf 18 (17, 20) 76 (63, 88) 26 (14, 38) 0.48 (0.23, 0.74) 0.16 (0.09, 0.23)
a

Inhibition experiments were conducted in triplicate. All data points were used to fit the data to eq 3. The data are reported as the values with the lower and upper 95% confidence limits.