Table 3.
Toxicity of HNL, TMZ, and BLM with Select Combinations with Cancer Cell Linesa
| tested compound | supplemented with | −log(IC50) (95% CI) | |
|---|---|---|---|
|
| |||
| 24 h | 72 h | ||
| GM12878 | |||
| TMZ | >3 | 3.5 (3.4, 3.7) | |
| TMZ | HNL | >3 | 3.9 (3.8, 4.1) |
| HNL | >3 | 4.7 (4.6, 4.9) | |
| HNL | TMZ | >3 | 4.9 (4.8, 5.1) |
| BLM | 4.1 (3.91, 4.3) | 4.1 (4.0, 4.3) | |
| BLM | HNL | 4.2 (4.0, 4.3) | 4.20 (4.0, 4.4) |
| HNL | 3.9 (3.7, 4.0) | 4.0 (3.8, 4.1) | |
| HNL | BLM | 3.9 (3.8, 4.1) | 4.0 (3.9, 4.1) |
| A549 | |||
| TMZ | 3.4 (3.3, 3.5) | 3.6 (3.4, 3.7) | |
| TMZ | HNL | 3.6 (3.5 3.7) | 4.1 (4.0, 4.2) |
| HNL | 4.6 (4.4, 4.7) | 4.0 (3.9, 4.0) | |
| HNL | TMZ | 4.9 (4.7, 5.0) | 4.1 (4.0, 4.2) |
| BLM | 4.8 (4.7, 5.0) | 5.2 (5.0, 5.3) | |
| BLM | HNL (10 μM) | 5.30 (5.18, 5.43) | 5.6 (5.5, 5.7) |
| HNL | 4.6 (4.5, 4.8) | 4.8 (4.6, 4.9) | |
| HNL | BLM (10 μM) | 5.0 (4.8, 5.1) | 5.3 (5.2, 5.4) |
| MCF7 | |||
| TMZ | 3.3 (3.2, 3.4) | 3.7 (3.6, 3.8) | |
| TMZ | HNL (10 μM) | 3.7 (3.6, 3.8) | 3.9 (3.7, 4.0) |
| HNL | 4.7 (4.6, 4.8) | 4.8 (4.7, 4.9) | |
| HNL | TMZ (250 μM) | 4.9 (4. 8, 5.0) | 5.0 (4.9, 5.1) |
| BLM | 4.9 (4.8, 5.0) | 5.3 (5.2, 5.4) | |
| BLM | HNL (10 μM) | 6.0 (5.7, 6.2) | 6.3 (6.1, 6.6) |
| HNL | 4.8 (4.7, 4.8) | 4.9 (4.8, 5.0) | |
| HNL | BLM (10 μM) | 5.6 (5.4, 5. 7) | 5.6 (5.4, 5.8) |
| PANC1 | |||
| TMZ | 3.5 (3.4, 3.7) | 3.8 (3.7, 3.9) | |
| TMZ | HNL (10 μM) | 3.9 (3.8, 4.0) | 4.3 (4.2 4.4) |
| HNL | 4.7 (4.5, 4.9) | 5.1 (5.0, 5.3) | |
| HNL | TMZ(250 μM) | 5.0 (4.9, 5.1) | 5.3 (5.2, 5.4) |
| BLM | 4.9 (4.7, 5.1) | 5.2 (5.0, 5.3) | |
| BLM | HNL (10 μM) | 5.9 (5.7, 6.2) | 6.09 (5.9, 6.3) |
| HNL | 4.6 (4.5, 4.8) | 4.8 (4.7, 4.9) | |
| HNL | BLM (10 μM) | 5.5 (5.3, 5.7) | 5.6 (5.4, 5. 8) |
| UACC903 | |||
| TMZ | 3.2 (3.1, 3.3) | 3.5 (3.4, 3.7) | |
| TMZ | HNL (10 μM) | 3.6 (3.5, 3.8) | 3.9 (3.8, 4.1) |
| HNL | 4.6 (4.5 4.7) | 4.8 (4.7, 4.9) | |
| HNL | TMZ(250 μM) | 4.8 (4.7, 4.9) | 5.0 (4.9, 5.1) |
| BLM | 4.9 (4.7, 5.0) | 5.22 (5.1, 5.4) | |
| BLM | HNL (10 μM) | 5.5 (5.4, 5.7) | 6.3 (6.0, 6.5) |
| HNL | 4.6 (4.47, 4.8) | 4.8 (4.65, 4.9) | |
| HNL | BLM (10 μM) | 5.1 (5.0 5.2) | 5.4 (5.2, 5.5) |
a
The −log(EC50) values were determined for the tested compound alone and in the presence of 10 μM HNL, 10 μM BLM, or 250 μM TMZ. The experiments were conducted in triplicate. All data points were used to fit the data to eq 3. The data are reported as the value with the lower and upper 95% confidence limits.