Table 2.
Molecular and morphological features of breast cancer cell line subtypes. The panels of mRNAs, miRNAs, and protein markers used for the identification of each breast cancer cell line subtype, as well as their morphological differences are summarized. In triple negative cell lines, the markers shared between sublines A and B are presented separately in the mRNA and protein columns. The literatures where such information is derived are provided right after.
| Cell line subtype | mRNA | miRNA 1 | Protein | Morphology | ||||
|---|---|---|---|---|---|---|---|---|
| Luminal | ER, GATA3, KRT19/KRT8/KRT18, XBP1, PBX1, ZNF278, SPDEF, CRABP2, MUC1, FOXA1, MYB, RET, EGR3, TFF1, HER3, TOB1, TFF31, 5, 7, 8 | hsa-miR-501-5p hsa-miR-202 hsa-miR-760 hsa-miR-626 |
ER, GATA3, KRT19 7 | More differentiated; tight cell-cell junctions 5 |
||||
| HER2 positive | HER2, GRB7, PERLD1, STARD3, C17ORF377 | hsa-let-7b hsa-miR-640 hsa-miR-200c hsa-miR-378 hsa-miR-141 hsa-miR-196a hsa-miR-29c hsa-miR-18a* |
HER2 | Breakdown of cell-cell junctions 10 | ||||
| Triple negative | Triple negative A (Basal A) |
EGFR CAV1/2 MSN ETS1 5, 7, 8 |
KRT4/5/6A/6B/13/14/15/16/17, ITGA6, ITGB4/6, LAMB3, LAMC2, TRIM29, S100A2, SLPI, LYN, ANXA8, COL17A1, BNC1, MET, CD133, GABRK, VTCN1, BST2, FABP7, CD10/14/58/59, 1, 7, 8 | hsa-miR-492 hsa-miR-26b hsa-miR-617 hsa-miR-155 |
EGFR CAV1/2 MSN ETS1 7 |
KRT5/6 CD10 MET 7 |
Core basal-like | |
| Triple negative B (Basal B) |
VIM, SPARC, FN1, FBN1, HAS2, PRG1, COL3A1, COL6A1/2/3, COL8A1, MMP2/14, TIMP1, CTSC, PLAU, PLAUR, AXL, PLAT, CD24(-), CD44, TGFBR2, SERPINE1/2, TGFB15, 7, 8 | hsa-miR-22 hsa-miR-532-3p hsa-miR-125b hsa-miR-501-5p hsa-miR-155* |
CD44 7 | Least differentiated and most stemness; more mesenchymal-like appearance and highly invasive 5 | ||||