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. 2017 Sep 12;8(16):3154–3165. doi: 10.7150/jca.20086

Figure 4.

Figure 4

Down-regulation of FAM83B in PDAC cells leads to G1/S phase arrest and inhibition of cell proliferation and tumor growth. A. GSEA in GSE71729 tumor samples (median split according to FAM83B expression) indicated that 'CELL-CYCLE-PROCESS' pathway was enriched in patients with FAM83B-higher expression versus patients with FAM83B-lower expression. B. Knockdown efficiency was confirmed by real-time PCR in PANC-1 and AsPC-1 cell line. C. Down-regulation of FAM83B increased cell population at G0/G1 phase but decreased at S/G2 phase by flow cytometry analysis in PANC-1 and AsPC-1 cells. D. Western blot indicated down-regulation of FAM83B reduced the protein expression of CDK4, CDK6, cyclin D1, and enhanced the expression of p21Cip1. GAPDH was used as a loading control. E. The cell counting kit-8 assay indicated cell proliferation was blocked when FAM83B was reduced in PANC-1 and ASPC-1 cells.*p<0.05; ** p<0.01. The experiment was performed in six replicate wells for each group and all the experiments were conducted in triplicate. Error bars represented SEM. F-G. Down-regulation of FAM83B significantly reduced the PDAC tumor volume in the subcutaneous xenograft model (p=0.0194). Error bars represented SEM.