Figure 2.

Systemic activation of the immune system after administration of GD5. Tumor-bearing mice were treated intraperitoneally with 2.5 mg/kg GD5. After 2 and 4 h, splenocytes were harvested and stained with cell surface marker. The expression of CD3⁺CD69⁺ and CD19⁺CD69⁺ cells (A), CD11c⁺CD86⁺ and CD11c⁺CD80⁺ cells (B) were analyzed by flow cytometry. *p < 0.05, **p < 0.01, one-way ANOVA. Induction of systemic cytokines (C) were measured in the plasma. The serums were collected after 2, 4 and 24 h post GD5 treatment and analyzed by ELISA assay (5 mice/group).