Table 3.
Sequence analysis of GPC3 specific CTLs sorted from PBMCs.
| No. | BV | BD | BJ | CDR3 amino acids | frequency† | same sequence with TIL clone |
|---|---|---|---|---|---|---|
| 1 | TRBV5–1 | TRBD2 | TRBJ2–7 | CASQQSSGVAIHEQYF | 3/77 (3.9%) | TIL clone 1 |
| 2 | TRBV5–1 | TRBD2 | TRBJ2–3 | CASSVTSGRTHTDTQYF | 10/77 (13.0%) | |
| 3 | TRBV5–4 | TRBD1 | TRBJ1–3 | CASSPGTFSGNTIYF | 12/77 (15.6%) | |
| 4 | TRBV6–1 | TRBD1 | TRBJ2–7 | CASSRPLLGGGLYEQYF | 15/77 (19.5%) | TIL clone 2 |
| 5 | TRBV9 | TRBD1 | TRBJ1–2 | CASRGTGSMYGYTF | 3/77 (3.9%) | |
| 6 | TRBV9 | TRBD2 | TRBJ2–1 | CASSVGSGGGNEQFF | 8/77 (10.4%) |
†
Frequency indicate number of cells that have same TCR sequence in 77 sorted GPC3-dextramer+ cells from PBMCs.