Table 1.
Polymerase Chain Reaction Primers and Annealing Temperatures Used for the Various Genes
| Primer | Sequence (5′-3′) | Products sizes (bp) | Annealing (°C) |
|---|---|---|---|
| blaTEM | Fw- AGATTTCATCTTTGATTCTTGG | 868 | 45 |
| Rv- AATTGATTCTTTAGCATCTGG | |||
| blaSHV | Fw- CCAGCCAACTATGGCGGAATC | 822 | 53 |
| Rv- CCTGTCGCAAGATCGACTGTA | |||
| blaCTX-M | Fw- ATGTGCAGCACCAGTAAAGT | 542 | 53 |
| Rv- ACCGCGATATCGTTGGTGG | |||
| blaCTX-M-15 | Fw- AAAAATGATTCGGCTCCAGAA | 483 | 54 |
| Rv- TGCCAGATTCGCTCTCAAAG | |||
| Mdh | Fw- GTTTAACGTTAACGCCGGT | 275 | 65 |
| Rv- GGTAACACCAGAGTGACCA | |||
| gyrB | Fw- CGCGATAAGCGCGAC | 132 | 65 |
| Rv- ACCGTCTTTTTCGGTGGAA | |||
| O25pabBspe | Fw- TCCAGCAGGTGCTGGATCGT | 347 | 56 |
| Rv- GCGAAATTTTTCGCCGTACTGT |