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. 2017 Sep 13;65(11):627–636. doi: 10.1369/0022155417730021

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — (A) Fluorescence distribution patterns after cytofluorometric analysis of G6PD activity in RBCs of a homozygous or hemizygous G6PD-deficient individual. All RBCs have a low fluorescence intensity (region P2). (B) Heterozygous G6PD deficiency. One population of RBCs has a low fluorescence intensity (region P2), and another one has a high fluorescence intensity (region P3). (C) Healthy individual without G6PD deficiency. The majority of RBCs have a high fluorescence intensity (region P3). Only a few old RBCs with low G6PD activity are found in region P2. (D) Healthy individual with an asymmetric population of RBCs with a high fluorescence intensity (intensity in region P3 and a shoulder in region P2). A substantial amount of RBCs with low fluorescence intensity are localized in region P2. Without proper visual inspection of the figure, this patient is falsely classified as heterozygously G6PD deficient. Abbreviations: G6PD, glucose-6-phosphate dehydrogenase; RBCs, red blood cells.