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. 2016 Jul 13;16(5):559–569. doi: 10.1177/1533034616657977

Figure 2.

Figure 2.

Knockdown of immature colon carcinoma transcript 1 (ICT1) expression in non–small cell lung cancer (NSCLC) cells. A, The expression level of ICT1 was examined in 5 of lung cells line by quantitative real-time polymerase chain reaction (qRT-PCR). B, Representative images recorded under a fluorescence microscope of 95D and A549 cells infected with 2 shICT1 lentivirus for 96 hours. C, The 95D and A549 cells were transfected with negative control (NC) or shICT1 group for 5 days. Knockdown efficiency of ICT1 was confirmed by quantitative real-time PCR. D, Total cellular proteins were extracted 5 days after infection with NC or shICT1 group in 95D and A549 cells, and knockdown efficiency was assayed by Western blotting. **P < .01; ***P < .001; scale bar, 10 μm.