Skip to main content
. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Eur J Cancer. 2017 Jul 20;83:132–141. doi: 10.1016/j.ejca.2017.06.024

Figure 2. Sustained suppression of ABCC4 inhibits neuroblastoma cell growth and abolishes colony formation.

Figure 2

(A) Efficient inducible knockdown of ABCC4 is achieved in BE(2)-C cells following doxycycline (Dox) treatment, with each of three independent shRNA duplexes (72h timepoint). Actin is shown as a loading control. (B) Inducible ABCC4 knockdown inhibits BE(2)-C cell proliferation in short-term assays at 72h post-induction for each duplex compared its untreated control (t test). (C) Inducible ABCC4 knockdown abolishes the formation of BE(2)-C cell colonies for each duplex compared to its untreated control (t test). (D) Representative images for colony assays quantitated in panel C. Data in B and C show means and SD derived from three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.