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. 2017 Oct 3;3:17035. doi: 10.1038/celldisc.2017.35

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Copyright © 2017 The Author(s)

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SET7/9 and LSD1 regulate YY2 K247 methylation in cultured cells. (a) Short peptides containing unmodified (YY2 K247) or monomethylated K247 (YY2K247me1) were prepared for dot blot assay, followed by IB using anti-YY2K247pan (left panel) or anti-YY2K247me1 (right panel) antibody as indicated. (b) HeLa cells transfected with vectors expressing Flag-tagged YY2 (wt) or YY2 (K247R) were subjected to IB with anti-YY2K247me1 or anti-Flag antibody as indicated. (c) Control (wt), SET7/9 or LSD1 KO HeLa cells transfected with control vector or vector expressing Flag-tagged YY2 were subjected to IB with antibodies as indicated. Intensity of YY2K247me1 was quantified by using Image J and normalized values were shown as indicated. (d) HeLa cells transfected with vector expressing Flag-tagged YY2 in the presence or absence of SET7/9 were subjected to IP with anti-Flag antibody followed by IB with antibodies as indicated. Intensity of YY2K247me1 was quantified by using Image J and normalized values were shown as indicated. Intensity of YY2K247me1 was quantified by using Image J and normalized values were shown as indicated.