Figure 6.

YY2 K247 methylation is involved in YY2-regulated gene transcriptional program. (a–c) RNA prepared from control (wt), YY2, SET7/9 or LSD1 KO HeLa cells were subjected to RNA-seq analysis. Pie chart was used to display genes positively or negatively regulated by YY2 (a), SET7/9 (b) or LSD1 (c; P<0.001). (d) Venn diagram showing overlapping between YY2 and SET7/9-regulated gene programs. (e) Pie chart showing gene programs regulated by YY2 and SET7/9 in the same direction including positively and negatively regulated genes (P<0.001). (f) Venn diagram showing overlapping between gene programs negatively regulated by LSD1 and those positively regulated by YY2 and SET7/9 in common. (g) Genome browser view of RNA-seq as described in a–c for selected genes as indicated was shown. (h) RNA prepared from control (wt), YY2, SET7/9 or LSD1 KO HeLa cells was subjected to RT-qPCR analysis to examine mRNA levels of genes as indicated. Data shown were the relative fold change compared with control samples after normalization to actin (±s.e.m., *P<0.05, **P<0.01, ***P<0.001). (i) HeLa cells were transfected with control vector or vectors expressing YY2 (wt) or YY2 (K247R), followed by RT-qPCR analysis to examine mRNA levels of selected genes as indicated. Data shown were the relative fold change compared with control samples after normalization to actin (±s.e.m., *P<0.05, **P<0.01, ***P<0.001). (j) HeLa cells as described in i were subjected to IB analysis using antibodies as indicated.