Fig. 3.

Optogenetic kindlin-2 interaction with integrin β3 promotes endothelial cell adhesion and migration. (A,B) Cells expressing the indicated β3 and kindlin-2 constructs were seeded onto fibrinogen-coated wells and incubated with or without pulsed blue light illumination for 1 h. After washing and fixation, attached cells were stained with Crystal Violet and cell adhesion was quantified as described in the Materials and Methods. Total cell input was assigned a value of 100%. (C) Cell migration across fibrinogen-coated Transwells. Migration of cells in the dark was arbitrarily assigned a fold change as 1. Data represent mean±s.e.m. from at least three experiments. *P<0.05; **P<0.01 (paired Student's t-test).