Fig. 6.

The effects of kindlin-2 interaction with β3 integrin on endothelial cells also require kindlin-2 interactions with other binding partners. Mutations were introduced into kindlin-2 as shown in Table 1 and expressed along with β3ΔRGT–GFP–LOVpep. (A) Cell migration across fibrinogen-coated Transwells. Migration of cells expressing β3ΔRGT–GFP–LOVpep and ePDZb1–mCherry–kindlin-2 without illumination was arbitrarily assigned a value of one. (B) Representative images and quantification of endothelial cell sprouting. Data represent mean±s.e.m. from four to five experiments. *P<0.05 (paired Student's t-test).