Fig. 7.

Optogenetic modulation of endothelial cell functions by kindlin-2 interaction with integrin β3 also requires fibrinogen and talin binding to β3. Point mutations, L746A or D119A, were introduced to create talin binding-defective (L746A) or ligand binding-defective (D119A) β3 mutants. Following expression of β3L746A/ΔRGT–GFP–LOVpep or β3D119A/ΔRGT–GFP–LOVpep along with ePDZb1–mCherry–kindlin-2 in endothelial cells, cell migration (A) and endothelial cell sprouting (B) were quantified. Data represent mean±s.e.m. from three experiments. *P<0.05; N.S., not significant (paired Student's t-test).