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. 2017 Oct 15;130(20):3455–3466. doi: 10.1242/jcs.207514

Fig. 4.

Fig. 4.

LPP3 knockdown inhibits chemotaxis in uniform serum. (A) LPP3 knockdown (LPP3 siRNA 1) does not inhibit chemotaxis of melanoma cells in the presence of a serum gradient, as assessed by (i) chemotactic index or (ii) rose plot and spider plot (n>100 cells per experiment, two independent experiments). (B) LPP3 knockdown inhibits differential chemotaxis of melanoma cells in a uniform serum gradient, as shown when comparing (i) scrambled siRNA controls against cells targeted with two different siRNAs against LPP3 (ii, iii) (n>100 cells per experiment, four independent experiments; P<0.05 comparing clusters 1 and 2 of scrambled versus LPP3 siRNA 1 and 2, one-way ANOVA). (C) Chemotaxis of melanoma cells in a uniform serum gradient in the presence of either DMSO or 500 nM HA130 to inhibit autotaxin function, or in a uniform serum-free (SF) gradient (n>100 cells per experiment, three independent experiments).