Fig. 2.
Tumor formation by BAP-complex-depleted tissue. (A-F) Low-magnification views of whole larvae showing the imaginal disc overgrowth for the indicated transgene combinations. GFP-expressing tissue expands massively to fill the anterior of the animal. (G-J) Confocal micrographs of wing discs expressing the indicated combinations of UAS-transgenes. Discs were labeled with antibody to Matrix metalloprotease 1 (Mmp1, red) as well as UAS-GFP to mark the Yki-expressing tissue (green) and DAPI (blue) to outline the tissue. Mmp1 channel shown separately in gray below. (K,L) Confocal micrographs of wing discs expressing the indicated combinations of UAS-transgenes. xy section is shown in the central picture; xz section is shown in the bottom; and yz section is shown in the right side. Note that the tissue in K maintains the normal epithelial organization whereas the tumor in L is highly disorganized. Discs large (Dlg) is show in red and gray. DAPI labels the nuclei and is shown in blue and gray. GFP is shown in green. (M,O) Confocal micrographs of wing discs expressing the indicated combinations of UAS-transgenes. Viking (vkg)-GFP was used to label the basement membrane and is shown in green and gray. The basement membrane appears as a continuous layer in the disc in M, whereas it is more disorganized and in some parts has been degraded in the tumor in O. (N,P) Fluorescent images of whole flies, showing GFP-expressing allograft tissue in the host abdomen. Fragments of imaginal discs were implanted in the abdomen of a host female and allowed to grow for 2 weeks. (N) apGal4, UAS-Yki+UAS-GFP, 0/12 showed overgrowth; (P) apGal4, UAS-Yki+UAS-BrmRNAi, 16/19 showed overgrowth. Scale bars: 100 μm.