Fig. 3.

Obesity altered the expression of inflammatory mediators and adipokines in the plasma and metabolically active organs after stroke. Measurements were made in plasma from tail vein blood immediately prior to surgery (Pre) and at 24 h post-stroke (A). Measurements were also made in epididymal fat pad (B) and liver (C) homogenates taken from naïve animals or at 24 h post-stroke, and normalised to milligram of total protein as measured by bicinchoninic acid (BCA) assay. Cytokine (IL-6, TNFα, CCL2, CXCL1, G-CSF) concentrations were measured by CBA. ICAM-1, adiponectin and resistin concentrations were measured by ELISA. Data are means±s.e.m., n=10 for naïve and n=14 for stroke groups, *P<0.05 versus control ob/– for same treatment, ^#P<0.05 versus pre-surgery or naïve for same genotype using two-way ANOVA with Šídák correction for multiple comparisons. ND, not detected.