Fig. 4.

Hydrogen peroxide and aristolochic acid have no effect on extracellular matrix deposition in RPTECs. (A) Effect of H₂O₂ on extracellular matrix deposition as assayed using the immunofluorescence ECM assay. Epithelial cells were incubated for 6 days with indicated concentrations of H₂O₂ (white-filled bars). (B) As A, except aristolochic acid (AA) was used as the stimulus. Representative example from two independent experiments is shown. (C) mRNA for extracellular matrix component transcripts determined by qRT-PCR after 48 h in culture with AA or H₂O₂. Data shows mean±s.d. for four independent experiments.