Fig. 6.

Co-culture of fibroblasts and epithelial cells modulates extracellular matrix production. (A) Representative images of the matrix accumulated by epithelial cells and fibroblasts in monoculture, or co-culture at a 1:1 cell ratio (with equal total numbers of cells per well) when cultured for six days without stimulation. Cells have been stripped, matrix-fixed and immunostained for matrix components: fibronectin (green), collagen I and III (red), collagen IV (blue). Scale bars: 100 µm. (B) Quantification of immunofluorescence for extracellular matrix components for cells as in A with and without stimulation with 10 ng/ml TGFβ1. Graphs show mean of four replicate wells ±s.e.m., one representative experiment of three shown.