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. 2017 Aug 17;6(10):1445–1457. doi: 10.1242/bio.025957

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Xenopus laevis embryos express endogenous HCN4 channel during early development. Immunofluorescence analysis of whole Xenopus embryos for HCN4 channel protein at indicated stages of development. (i-vi) No primary antibody controls, (vii – xii) HCN4 immunofluorescence, (i, iii, v, vii, ix, xi) bright field images of immunofluorescent embryos, (ii, iv, vi, viii, x, xii) fluorescence images of immunofluorescence embryos. Xenopus embryos at the indicated stage of development showed a prominent HCN4 channel protein (n=15).