Fig. 2.

Vgll3 knockdown or overexpression impairs trigeminal placode and nerve formation. (A) Embryos injected with v3MO or v3MOsplicing (v3LMOsp and v3SMOsp, 20 ng each) exhibit reduced expression of islet1, neuroD and N-tubulin in the trigeminal placodes (arrowheads). (B) Overexpression of increasing amounts of vgll3 mRNA reduces islet1 and N-tubulin expression in stage 19 embryos. Double ISH shows no prominent overlapping staining between vgll3 (red) and N-tubulin (blue) (arrow). The injected side (indicated with asterisks) was traced by lacZ staining. Gene expression was assayed by ISH. Arrowheads indicate the trigeminal placodes. (C) Quantification of results. Images at the top of bars indicate v3LMOsp defects rescued with increasing amounts of vgll3 mRNA injections. Three independent experiments were performed. The number of embryos analysed is indicated on the top of each bar. Views are dorsal-anterior excepted for lateral views for stage 28 embryos.