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. 2017 Sep 4;6(10):1528–1540. doi: 10.1242/bio.026153

Fig. 4.

Fig. 4.

Vgll3 knockdown and overexpression do not affect CNC formation but block their migration. Embryos were injected with v3MO (40 ng or cMO) or vgll3 mRNA (1 ng, or lacZ mRNA) and analysed at different stages for snail2, twist, PCNS or pcdh18 expression. Pharyngeal arches are indicated (a, anterior; br, branchial; hy, hyoid; ma, mandibular; p, posterior). Arrowheads indicate the mandibular branch of the trigeminal nerve. Vgll3 knockdown and overexpression block migration of CNC streams. Arrows indicate overlapping expression of vgll3 and snail2. White lines indicate the plane of agarose section. Asterisks indicate the injected side. Dashed lines indicate the midline of embryos. The oval indicates the lateral CNC stream. All views are dorsal-anterior except lateral views for stage 25 and 28 embryos. Quantification of results is shown in the lower panels. Three independent experiments were performed. The number of embryos analysed is indicated at the top of each bar.