Fig. 4.

UBA1 depletion perturbs VEGFR2 endosome-lysosome dynamics. (A) Endothelial cells transfected with non-targeting or UBA1 siRNA were processed for immunofluorescence microscopy using antibodies to VEGFR2 (green), EEA1 (red), CD63 (red) or LAMP2 (red) followed by fluorescent species-specific secondary antibodies. Nuclei were stained with DNA-binding dye, DAPI (blue). Scale bar: 70 μm. (B) Quantification of VEGFR2 co-distribution with early endosome (EEA1), late endosome (CD63) and lysosome (LAMP2) markers in endothelial cells transfected with non-targeting control siRNA or UBA1-specific siRNA. Error bars denote mean±s.e.m. (n≥3), with significance denoted as *P<0.05; analysed using two-way ANOVA.