Fig. 5.

Spindle angle and midbody placement in developing renal vesicles. (A-C′) Immunolabeling of microtubules with α-tubulin (red) shows the mitotic spindle (A,B) and the midbody (C), which contains concentrated microtubules, in developing nephron tubules. The apical surface is marked by Par6b (blue) and the lumen (L) is indicated. Spindles appear parallel to basal and apical surfaces in a renal vesicle (A) and later tubule (B). Midbodies are apically located (C, arrow). A′-C′ are high magnifications of the boxed areas in A-C. Scale bars: 10 µm.