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. 2017 Oct 1;144(19):3454–3464. doi: 10.1242/dev.149500

Fig. 1.

Fig. 1.

Imp and Syp regulate non-MB NB decommissioning. (A-D″″) Ectopic Imp or Syp-depletion prolongs NB life into the adult. Composite confocal images of control (CTRL; A-A″″), Syp RNAi (B-B″″), Imp gain of function (GOF; C-C″″), and Syp/Imp-depleted (D-D″″) fly brains at specific developmental times, immunostained for GFP (green), Mira (magenta) and phospho-Histone H3 (pH3, blue). Transgenes were driven by dpnEE-GAL4 in the NBs of central brain. Yellow arrows indicate the MB NBs. Insets show the boxed areas at higher magnification. Scale bar: 50 µm (10 µm in inset). (E) Quantification of NB size in the anterior region of the fly brain (measured by the diameter of Mira-labeled NBs, mean±s.d., n=6 brains). (F) Syp depletion prolongs NB Imp expression. Representative confocal images of 8 h APF fly brains immunostained for Imp (magenta), GFP (green) and Dpn (blue) in control and Syp depletion conditions/experiments driven by dpnEE-Gal4. Scale bar: 10 µm. (G) Imp gain of function did not affect Syp expression. Representative confocal images of 8 h APF fly brains immunostained for Syp (magenta), GFP (green) and Dpn (blue) in control and dpnEE-Gal4-driven Imp gain-of-function conditions/experiments. In F and G, NBs with a maximum diameter at the given focal plane are circled. Scale bar: 10 µm.