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. 2017 Oct 1;144(19):3454–3464. doi: 10.1242/dev.149500

Fig. 5.

Fig. 5.

The mediator complex functions downstream of Imp to induce NB shrinkage. (A-B″″) Med12 depletion blocks the inhibition of NB shrinkage caused by persistent Imp expression. Composite confocal images of Syp and Med12 joint knockdown (A) and Imp gain of function combined with Med12 knockdown (B) driven by dpnEE-Gal4. Brains were dissected at specific developmental ages and immunostained for GFP (green), Mira (magenta) and phospho-Histone H3 (pH3, blue). Insets show the boxed areas at higher magnification. Scale bar: 50 µm (10 µm in inset). (C-E) The mediator complex acts downstream of Imp to regulate MB NB shrinkage. Representative confocal images of 48 h APF fly brains immunostained for GFP (green), Dpn (magenta) and Pros (blue) in Med12 depleted (C) and combined Imp-depleted with Med12 gain-of-function conditions (D) driven by Gal4-OK107. Diameter of MB NBs at 48 h APF (mean±s.d., n=6 brains) is indicated at the top right of each panel. Scale bar: 20 µm. (E) Mean MB NB diameter (±s.d., n=6 brains) of flies at different developmental stages with transgenic manipulations driven by GAL4-OK107. *P<0.05, **P<0.01 (Student's t-test).